Lab references

Vitamins, Minerals, and Nutrient Status

25-hydroxyvitamin D, total — lab reference

<10 severe deficiency; 10-19 mild-moderate deficiency. Toxicity assessment requires clinical context.

Updated Sep 23, 2026 · Educational reference

Purser Wellness approved rule

Unit: ng/mL

Male: 20-50 optimum

Female: 20-50 optimum

How to read this marker

<10 severe deficiency; 10-19 mild-moderate deficiency. Toxicity assessment requires clinical context.

The wider testing context

Serum values do not always reflect tissue status. Specimen handling, fasting, recent supplements, inflammation, and assay method are especially important.

Using this reference

This is the approved adult, nonpregnant baseline unless the rule explicitly states otherwise. Keep the performing laboratory’s reference interval separate from this Purser Wellness rule. Match units and review the specimen, method, timing, medicines and relevant clinical context before interpreting a result. These pages do not calculate a personal high/low status or prescribe treatment.

A question to bring to your visit

Which parts of the 25-hydroxyvitamin D, total rule apply to my report’s method, units and collection conditions, and what other findings should we review alongside it?

Approved by Dr. Daniel Purser, MD · PW-LABS-1.0. The source laboratory’s interval remains separate.

Names you may see

25-hydroxyvitamin D, total · 25-hydroxyvitamin D